pcr Pt.2-DJMINION
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TXT pcr Pt.2-DJMINION 文本歌词
Then move on next slide we going to talk about Process
Step 1 - Denaturation
The solution contained in the tube is heated to at least 94°C (201.2°F) using a thermal cycler.
breaks the hydrogen bonds of the original DNA sample and separates the DNA into single strands
Step 2 - Annealing
then cooled to between 50 to 60°C (122 to 140°F)
allowing the DNA primers and the DNA polymerase enzyme to bind to the individual strands of DNA
the nucleotides (A, T, C, G) pair with the individual separated strands of DNA that resulted from the heating process.
Step 3 - Extension
Once joined together, they form a new complementary strand of DNA (termed extension of the DNA)
Material
dNTP mixtures
Amplification buffer
Primer
Template DNA
Taq DNA polymerase
Mg2 + solution
double distilled water
Clinical application
PCR takes a few pieces of DNA and replicates it millions of times using thermal cycling and primers. The it can increase the volume and diagnose cancer, genetic diseases, and some infectious diseases
detecting viral and bacterial infections,
the technician will use a tissue sample from the patient and mix it with a primer that targets the viruses or bacteria.
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