pcr Pt.2 (伴奏)-DJMINION

歌手 : DJMINION

专辑 : pcr

语种 : 英语

时长 : 03:00

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TXT pcr Pt.2 (伴奏)-DJMINION 文本歌词

Then move on next slide we going to talk about Process

Step 1 - Denaturation

The solution contained in the tube is heated to at least 94°C (201.2°F) using a thermal cycler.

breaks the hydrogen bonds of the original DNA sample and separates the DNA into single strands

Step 2 - Annealing

then cooled to between 50 to 60°C (122 to 140°F)

allowing the DNA primers and the DNA polymerase enzyme to bind to the individual strands of DNA

the nucleotides (A, T, C, G) pair with the individual separated strands of DNA that resulted from the heating process.

Step 3 - Extension

Once joined together, they form a new complementary strand of DNA (termed extension of the DNA)

Material

dNTP mixtures

Amplification buffer

Primer

Template DNA

Taq DNA polymerase

Mg2 + solution

double distilled water

Clinical application

PCR takes a few pieces of DNA and replicates it millions of times using thermal cycling and primers. The it can increase the volume and diagnose cancer, genetic diseases, and some infectious diseases

detecting viral and bacterial infections,

the technician will use a tissue sample from the patient and mix it with a primer that targets the viruses or bacteria.